Figure 3.

Characterization of new PdL mutations. (a) PdL(3)3E36 (Red herring and encore (enc)); (b) PdL(2)4G14 (VhaSFD); (c) PdL(3)2C33 (Sugar baby); (d) PdL(3)8S64 (filamin); (e) PdL(3)8S25 (four wheel drive (fwd)); (f) PdL(3)8R128 (CTP:phosphocholine cytidylyltransferase-1 (Cct1)). The molecular organization of each mutated gene is diagrammed. The PdL insertion is indicated by a triangle, with an arrow indicating the direction of transcription of the PdL tet-on promoter. Transcript start sites and ATG translation start codons are marked by arrows. The location of PdL insertions, transcription start sites, ATG translation start sites, and transcript termination sites are indicated by genomic scaffold numbers. mRNA was isolated from whole adult male flies cultured ±DOX and analyzed by northern blot. Hybridization with ribosomal protein gene Rp49 probe was used as a loading control. The locations of gene-specific DNA probes used for northern analyses are indicated. Transcript sizes were estimated using RNA markers run in adjacent lanes (data not shown). Two representative survival curves are presented for each line.