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Characterizing the stress/defense transcriptome of Arabidopsis

Ramamurthy Mahalingam1, AnaMaria Gomez-Buitrago2, Nancy Eckardt4, Nigam Shah3, Angel Guevara-Garcia5, Philip Day3, Ramesh Raina2* and Nina V Fedoroff1*

Author affiliations

1 Life Sciences Consortium, Pennsylvania State University, State College, PA 16802, USA

2 Department of Biology, Pennsylvania State University, State College, PA 16802, USA

3 Integrative Biosciences Graduate Degree Program, Pennsylvania State University, State College, PA 16802, USA

4 American Society of Plant Biologists, Rockville, MD 20855, USA

5 Instituto de BiotecnologĂ­a-UNAM, Av. Universidad No. 2001Col. Chamilpa, Cuernavaca, Morelos CP 62271, Mexico

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Citation and License

Genome Biology 2003, 4:R20  doi:10.1186/gb-2003-4-3-r20

Published: 18 February 2003

Abstract

Background

To understand the gene networks that underlie plant stress and defense responses, it is necessary to identify and characterize the genes that respond both initially and as the physiological response to the stress or pathogen develops. We used PCR-based suppression subtractive hybridization to identify Arabidopsis genes that are differentially expressed in response to ozone, bacterial and oomycete pathogens and the signaling molecules salicylic acid (SA) and jasmonic acid.

Results

We identified a total of 1,058 differentially expressed genes from eight stress cDNA libraries. Digital northern analysis revealed that 55% of the stress-inducible genes are rarely transcribed in unstressed plants and 17% of them were not previously represented in Arabidopsis expressed sequence tag databases. More than two-thirds of the genes in the stress cDNA collection have not been identified in previous studies as stress/defense response genes. Several stress-responsive cis-elements showed a statistically significant over-representation in the promoters of the genes in the stress cDNA collection. These include W- and G-boxes, the SA-inducible element, the abscisic acid response element and the TGA motif.

Conclusions

The stress cDNA collection comprises a broad repertoire of stress-responsive genes encoding proteins that are involved in both the initial and subsequent stages of the physiological response to abiotic stress and pathogens. This set of stress-, pathogen- and hormone-modulated genes is an important resource for understanding the genetic interactions underlying stress signaling and responses and may contribute to the characterization of the stress transcriptome through the construction of standardized specialized arrays.