Genome Biology
Volume 4
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 ReviewStatistical tests for differential expression in cDNA microarray experimentsXiangqin Cui and Gary A Churchill  The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA  author email corresponding author email
Genome Biology 2003,
4:210doi:10.1186/gb-2003-4-4-210
Subject areas: Bioinformatics, Genome studies, Methods Abstract
Extracting biological information from microarray data requires appropriate statistical methods. The simplest statistical method for detecting differential expression is the t test, which can be used to compare two conditions when there is replication of samples. With more than two conditions, analysis of variance (ANOVA) can be used, and the mixed ANOVA model is a general and powerful approach for microarray experiments with multiple factors and/or several sources of variation. | 
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