Figure 4.

Ouput of GoCharts. A) A bar chart showing the distribution of differentially expressed genes among Gene Ontology (GO) Biological Processes. Parameters were set to GO level 2, a hit threshold of five, and output was sorted by hit count. Blue bars are linked to additional annotation data shown in figure 4B. B) Selecting the blue bar in figure 4A corresponding to 'response to stress' opens an HTML table showing the LocusLink, Gene Name, Current Classification, and Other Classification data for the genes in that category. C) This subset of genes involved in 'stress response' was further characterized by selecting GO Molecular Function, GO level 3, a hit threshold of 2, and sorted by hit count. Selecting the 'Chart Values' button creates a new histrogram revealing that 16 of the 35 stress response genes possess cytokine activity.