A model of the stages of bacterial biofilm development. At stage 1, the bacterial cells attach reversibly to the surface. Then, at stage 2, the cells attach irreversibly, a step mediated mainly by exopolymeric substances, and the cells lose their flagella-driven motility. At the next stage (3), the first maturation phase is reached, as indicated by early development of biofilm architecture. The second maturation phase is reached at stage 4 with fully mature biofilms, as indicated by the complex biofilm architecture. At the dispersion stage (5), single motile cells (dark cells on the figure) disperse from the microcolonies. Adapted from .
Sauer Genome Biology 2003 4:219 doi:10.1186/gb-2003-4-6-219