Figure 1.

Unsupervised hierarchical cluster analysis of genes and experiments of nine normal genital skin fibroblast lines (penile foreskin) and five AIS genital skin fibroblast lines (labia majora). Only transcripts whose log₂ red/green ratio differed from the mean expression level across all experiments by at least 1.1 in at least four experiments are displayed (620 cDNAs). The dendrogram of the array experiments (above and repeated on the left) reflects the similarity of the samples with respect to their gene-expression patterns. F, female external genitalia; M, normal male external genitalia; NORM, normal male control; AIS4, AIS with predominantly female phenotype (slight enlargement of the clitoris); CAIS, complete androgen-insensitivity syndrome; GSF, genital skin fibroblast. Increasing red intensity corresponds to increased gene-expression levels compared to the mean log₂ red/green ratio for each gene; increasing green intensity corresponds to decreased gene-expression levels. The scale ranges from -8 to +8 in log₂ space.