Proposed changes in the hypothetical protein YMR269W. (a) Multiple alignment of the translated +2 and +3 frames of the S. cerevisiae YMR269W region and the syntenic A. gossypii protein. The boxed sequence indicates the region of the potential shift from frame +3 to +2 as suggested by the multiple alignment. (b) S. cerevisiae YMR269W region. Light gray depicts the current annotation as available at SGD. Dark gray shows the proposed elongation of the frame +2 translation on the 5' end of the yeast YMR269W gene. (c) Domain organization of YMR269W proteins in S. cerevisiae and A. gossypii. While the current YMR269W protein sequence of S. cerevisiae carries only a nuclear localization signal, both the A. gossypii homolog and the proposed extended YMR269W protein have an additional G-patch domain, which has been described as a putative RNA-binding domain.
Brachat et al. Genome Biology 2003 4:R45 doi:10.1186/gb-2003-4-7-r45