Figure 5.

Southern analysis of the CHRNA7 duplication. Probe CHRNA7-PstI was used to demonstrate a 104 bp RFLP distinguishing the intact (548 bp band) and duplicated (444 bp band) CHRNA7 loci in the human genome (lane 1). A single band - corresponding to the intact CHRNA7 locus - is seen in PTR, PPA and GGO (lanes 2 and 3, 4 and 5, 6 and 7, respectively), indicating that the duplication of CHRNA7 was a recent human-specific event and that therefore the gene is single copy in the PTR genome.