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Highly Accessed Opinion

Comparing protein abundance and mRNA expression levels on a genomic scale

Dov Greenbaum1, Christopher Colangelo23, Kenneth Williams23* and Mark Gerstein24*

Author Affiliations

1 Department of Genetics, Yale University, New Haven, CT 06520-8114, USA

2 Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520-8114, USA

3 HHMI Biopolymer Laboratory and W. M. Keck Foundation Biotechnology Resource Laboratory, Yale University, New Haven, CT 06520-8114, USA

4 Department of Computer Science, Yale University, New Haven, CT 06520-8114, USA

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Genome Biology 2003, 4:117  doi:10.1186/gb-2003-4-9-117

Published: 29 August 2003

Abstract

Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.