Table 2

List of ORFs in E. coli with updated annotations based on sequence similarity

EC number

Bnum

Gene

Published annotation [12]

Suggested annotation


1.1.1.48

b1315

ycjS

Putative NADH-dependent dehydrogenase

D-Galactose 1-dehydrogenase

1.1.1.48

b1624

ydgJ

Putative NAD(P)-binding dehydrogenase

D-Galactose 1-dehydrogenase

1.1.1.48

b3440

yhhX

Putative NAD(P)-binding dehydrogenase

D-Galactose 1-dehydrogenase

1.1.1.5

b2426

ucpA

Putative oxidoreductase, NAD(P)-binding

Acetoin dehydrogenase, diacetyl reductase

1.1.1.5

b2137

yohF

Putative oxidoreductase

Acetoin dehydrogenase, diacetyl reductase

1.1.1.5

b4266

idnO

5-Keto-D-gluconate-5-reductase

Acetoin dehydrogenase, diacetyl reductase

1.2.1.19

b1385

feaB

Phenylacetaldehyde dehydrogenase

Aminobutyraldehyde dehydrogenase

1.2.1.19

b1444

ydcW

Putative aldehyde dehydrogenase

Aminobutyraldehyde dehydrogenase

1.2.1.19

b3588

aldB

Aldehyde dehydrogenase B (lactaldehyde dehydrogenase)

Aminobutyraldehyde dehydrogenase

1.2.1.24

b1385

feaB

Phenylacetaldehyde dehydrogenase

Succinate-semialdehyde dehydrogenase

1.2.1.24

b1444

ydcW

Putative aldehyde dehydrogenase

Succinate-semialdehyde dehydrogenase

1.2.1.24

b1415

aldA

Aldehyde dehydrogenase A, NAD-linked

Succinate-semialdehyde dehydrogenase

1.2.7.1

b1378

nifJ

Putative pyruvate-flavodoxin oxidoreductase

Pyruvate synthase

1.3.1.2

b2146

yeiT

Putative glutamate synthase

Dihydrothymine dehydrogenase

1.3.1.2

b2147

yeiA

Putative dihydropyrimidine dehydrogenase, FMN-linked

Dihydrothymine dehydrogenase

1.3.1.2

b2878

b2878

Putative oxidoreductase

Dihydrothymine dehydrogenase

1.3.99.3

b1695

ydiO

Putative acyl-CoA dehydrogenase

Acyl-CoA dehydrogenase

1.3.99.3

b0039

caiA

Putative acyl-CoA dehydrogenase, carnitine metabolism

Acyl-CoA dehydrogenase

1.3.99.3

b4187

aidB

Putative acyl-CoA dehydrogenase; adaptive response (transcription activated by Ada)

Acyl-CoA dehydrogenase

1.6.6.9

b3551

bisC

Biotin sulfoxide reductase

Trimethylamine-N-oxide reductase (TMAO reductase II)

1.6.6.9

b1587

b1587

Putative reductase

Trimethylamine-N-oxide reductase (TMAO reductase II)

1.6.6.9

b1588

b1588

Putative reductase

Trimethylamine-N-oxide reductase (TMAO reductase II)

1.6.99.2

b0046

yabF

Putative electron transfer flavoprotein-NAD/FAD/quinone oxidoreductase, subunit for KefC K+ efflux system

NAD(P)H dehydrogenase

1.6.99.2

b3351

yheR

Putative electron transfer flavoprotein-NAD/FAD/quinone oxidoreductase

NAD(P)H dehydrogenase

1.6.99.2

b0901

ycaK

Putative electron transfer flavoprotein-NAD/FAD/quinone oxidoreductase

NAD(P)H dehydrogenase

2.3.3.11 (formerly 4.1.3.9)

b2264

menD

Bifunctional modular MenD: 2-oxoglutarate decarboxylase and SHCHC synthase

2-Hydroxyglutarate synthase

2.3.3.11 (formerly 4.1.3.9)

b3671

ilvB

Acetolactate synthase I, large subunit, valine-sensitive, FAD and thiamine PPi binding

2-Hydroxyglutarate synthase

2.7.1.23

b2615

yfjB

Conserved protein

NAD+ kinase

2.7.1.23

b3916

pfkA

6-phosphofructokinase I

NAD+ kinase

2.7.2.1

b3115

tdcD

Propionate kinase/acetate kinase II, anaerobic

Acetate kinase (ackB)

2.7.8.2

b1408

b1408

Putative enzyme

Diacylglycerol cholinephosphotransferase

2.8.1.2

b2521

sseA

Putative sulfurtransferase

3-Mercaptopyruvate sulfurtransferase

2.8.1.2

b1757

ynjE

Putative thiosulfate sulfur transferase

3-Mercaptopyruvate sulfurtransferase

2.8.3.8

b2222

atoA

Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit

Acetate CoA-transferase

2.8.3.8

b2221

atoD

Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit

Acetate CoA-transferase

2.8.3.8

b1694

ydiF

Putative acetyl-CoA:acetoacetyl-CoA transferase alpha subunit

Acetate CoA-transferase

3.1.1.31

b0678

nagB

Glucosamine-6-phosphate deaminase

6-Phosphogluconolactonase (Pgl)

3.1.1.31

b3718

yieK

Putative isomerase

6-Phosphogluconolactonase (Pgl)

3.1.1.31

b3141

agaI

Putative galactosamine-6-phosphate isomerase

6-Phosphogluconolactonase (Pgl)

3.2.1.68

b3431

glgX

Glycosyl hydrolase

Isoamylase

3.5.1.2

b1524

yneH

Putative glutaminase

Glutaminase A or B

3.5.1.2

b0485

ybaS

Putative glutaminase

Glutaminase A or B

3.6.1.19

b2954

yggV

Conserved hypothetical protein

Nucleoside-triphosphate (ITP) diphosphatase

3.6.1.22

b3996

yjaD

Conserved hypothetical protein, MutT-like protein

NAD+ diphosphatase

3.6.1.5

b3615

yibD

Putative glycosyltransferase

Inosine triphosphate diphosphatase

3.6.1.6

b3614

yibQ

Conserved hypothetical protein

Nucleoside (IDP) diphosphatase

4.1.1.39

b3615

yibD

Putative glycosyltransferase

Ribulose-bisphosphate carboxylase (2-phosphoglycolate forming)

4.1.2.10

b0311

betA

Choline dehydrogenase, a flavoprotein

Hydroxynitrile lyase

4.1.2.10

b3168

infB

Protein chain initiation factor IF-2

Hydroxynitrile lyase

4.2.1.66

b3022

ygiU

Conserved protein

Cyanide hydratase

6.2.1.12

b4069

acs

Acetyl-CoA synthetase

Hydroxycinnamate-CoA ligase

6.2.1.12

b1701

ydiD

Putative CoA-dependent ligase

Hydroxycinnamate-CoA ligase

6.2.1.12

b2836

aas

bifunctional multimodular Aas: 2-acylglycerophospho-ethanolamine acyl transferase

Hydroxycinnamate-CoA ligase

6.2.1.4

b0728

sucC

Succinyl-CoA synthetase, beta subunit

Succinate-CoA ligase (ITP forming)

6.2.1.4

b0729

sucD

Succinyl-CoA synthetase, alpha subunit, NAD(P) binding

Succinate-CoA ligase (ITP forming)


Annotations shown in bold arose from analyzing the network gaps in iJR904.

Reed et al. Genome Biology 2003 4:R54   doi:10.1186/gb-2003-4-9-r54

Open Data