Table 2

Whole genome comparison of real versus randomized miRNAs against the complete genomes of D. melanogaster and D. pseudoobscura

Total hits

Total conserved hits

1 site

≥ 2 sites


73 D. melanogaster miRNAs (A)

6,864

589

556

33

73 Random miRNAs (B)

5,152

204

201

3

Standard deviation (100 experiments)

± 132

± 43

± 40

± 3

Ratio (A/B)

2.9

2.8

11.0

Estimated false positives (%)

35%

36%

9%


Detected conserved hits (especially those with multiple detected sites in the 3' UTR) are significantly over-represented (2.8× and 11× as many cases, on average, respectively) in analyses with actual miRNAs compared to randomly shuffled miRNAs. The thresholds used for this analysis were S: 100; ΔG: -19 kcal/mol; ID: 70%.

Enright et al. Genome Biology 2003 5:R1   doi:10.1186/gb-2003-5-1-r1

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