Table 2 |
||||
|
Whole genome comparison of real versus randomized miRNAs against the complete genomes of D. melanogaster and D. pseudoobscura |
||||
|
Total hits |
Total conserved hits |
1 site |
≥ 2 sites |
|
|
|
||||
|
73 D. melanogaster miRNAs (A) |
6,864 |
589 |
556 |
33 |
|
73 Random miRNAs (B) |
5,152 |
204 |
201 |
3 |
|
Standard deviation (100 experiments) |
± 132 |
± 43 |
± 40 |
± 3 |
|
Ratio (A/B) |
2.9 |
2.8 |
11.0 |
|
|
Estimated false positives (%) |
35% |
36% |
9% |
|
|
|
||||
|
Detected conserved hits (especially those with multiple detected sites in the 3' UTR) are significantly over-represented (2.8× and 11× as many cases, on average, respectively) in analyses with actual miRNAs compared to randomly shuffled miRNAs. The thresholds used for this analysis were S: 100; ΔG: -19 kcal/mol; ID: 70%. |
||||
|
Enright et al. Genome Biology 2003 5:R1 doi:10.1186/gb-2003-5-1-r1 |
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