Table 2 |
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|
Summary for the Heidelberg Collection R1 |
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| Total |
Heidelberg Predictions |
BDGP Release 2/ BDGC R1 |
Other |
Common predictions |
|
|
|
|||||
| Heidelberg Collection R1 |
21,396 |
7,464 |
483 |
71 |
13,378 |
| Heidelberg PrimerSet |
21,306 |
7,463 |
442 |
65 |
13,336 |
| Heidelberg FlyArray |
20,948 |
7,319 |
425 |
62 |
13,142 |
| Expressed during development |
13,927 (66.5%) |
3,497 (47.8%) |
232 (54.6%) |
52 (83.9%) |
10,146 (77.2%) |
| Validation by RT-PCR |
386/478 (80.8%) |
334/424 (78.8%) |
ND |
ND |
52/54 (96.3%) |
|
|
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|
The Heidelberg Collection R1 resulted from the combination of our novel annotation with the published BDGP Release 2 annotation and the sequences of the BDGC R1 clones. The PrimerSet includes only those annotations for which we could successfully design primer pairs, and likewise, the Heidelberg FlyArray sums up the annotations that are included on our novel microarray. The next row presents the results of the developmental profiling; numbers given in parentheses are the percentage of annotations represented on the array that scored positive. The last row shows the validation rate of the microarray results by RT-PCR (amplicon length ≤750 bp). |
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|
Hild et al. Genome Biology 2003 5:R3 doi:10.1186/gb-2003-5-1-r3 |
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