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Table 4
*Expression patterns obtained by in situ* hybridization**
Name	GenBank accession number	Pattern	Evidence	Chromosome	Comments

HDC00027	BK003260	Ectoderm	ag	2L	-
HDC00627	BK003299	Ectoderm	-	2L	-
HDC00658	BK003302	Cellular blastoderm subset, salivary glands	-	2L	-
HDC00966	BK003326	Cellular blastoderm subset	-	2L	Intron CG11030
HDC00979	BK003327	Yolk nuclei	-	2L	-
HDC02005	BK003369	Maternal, subset of cells, embryonic large intestine	dp	2L	-
HDC02009	BK003370	Protocerebrum primordium, trunk mesoderm primordium	dp	2L	-
HDC02141	BK003388	Embryonic gut, cells in the head (stage 10/11)	-	2L	-
HDC02262	BK003403	Weak signal	dp	2L	-
HDC02272	BK003405	Weak signal	dp	2L	-
HDC02494	BK003424	Mesoderm anlage	dp	2L	Intron CG15288
HDC02527	BK003429	Salivary glands	dp	2L	-
HDC02528	BK003430	Protocerebrum primordium, anterior midgut primordium	dp	2L	-
HDC02634	BK003455	Cellular blastoderm subset	dp	2L	-
HDC02764	BK003493	Cellular blastoderm, ubiquitous, salivary glands	dp, EST	2L	Intron CG4838
HDC03057	BK003539	Maternal, blastoderm, ubiquitous, gut	dp, EST	2L	Intron CG5803 (overlap)
HDC03960	BK003614	Trunk mesoderm anlage, head mesoderm primordium	dp	2R	Opposite strand to CG17921 (overlap)
HDC04256	BK003630	Subset of mesoderm, gonads	dp	2R	-
HDC05090	BK003664	Subset of cells (procephalic ectoderm primordium?), midgut	-	2R	-
HDC05183	BK003670	Ubiquitous	dp	2R	-
HDC05573	BK003699	Midgut, central nervous system	dp, EST	2R	-
HDC06000	BK003754	Cellular blastoderm excluding ventral structures	dp	2R	Intron CG12369, same staining as HDC05999
HDC06241	BK003785	Ventral ectoderm anlage, trunk mesoderm anlage	dp	2R	-
HDC06636	BK003845	Maternal	dp, EST	2R	-
HDC07387	BK003934	Maternal, subset of cells until stage 12	-	2R	-
HDC07791	BK001850	Weak, ubiquitous at 4-8 h	-	3L	-
HDC08265	BK001908	Subset of cells	-	3L	-
HDC08749	BK001956	Weak, ubiquitous at 4-8 h	dp	3L	-
HDC09080	BK002002	Salivary glands	dp	3L	-
HDC09253	BK002020	Posterior spiracles, ectoderm	dp	3L	-
HDC09513	BK002067	Weak, ubiquitous at 4-8 h	dp	3R	-
HDC10019	BK002122	Salivary gland primordium, salivary glands	-	3L	Intron CG10741
HDC10028	BK002123	Ventral nerve cord	dp	3L	Intron CG12478
HDC10120	BK002139	Trunk mesoderm anlage, cuprophilic cells	-	3L	Intron CG 17697
HDC10292	BK002156	Lateral stripes blastoderm, third wave of neuroblasts	ag	3L	Predicted in 2.0 as CG17014
HDC10646	BK002195	Pole plasm, trunk mesoderm, salivary glands, embryonic midgut	dp	3L	-
HDC10913	BK002212	Anterior midgut primordium, posterior midgut primordium	dp	3L	Intron CG11614 (opposite strand)
HDC11249	BK002252	Malpighi, gonads	dp	3L	Intron CG32432 (opposite strand)
HDC11512	BK002283	Weak, ubiquitous at 4-8 h	dp	3L	-
HDC11876	BK002318	Weak, ubiquitous at 4-8 h	dp, EST	3R	Intron CG12163 (opposite strand)
HDC11908	BK002321	Ventral nerve cord, embryonic central nervous system	dp	3R	-
HDC12497	BK002400	Weak, ubiquitous at 4-8 h	-	3R	-
HDC12511	BK002404	Ectoderm	dp	3R	-
HDC12925	BK002446	Weak, ubiquitous at 4-8 h	EST	3R	-
HDC13248	BK002490	Weak, ubiquitous at 4-8 h	-	3R	-
HDC13350	BK002511	Ectoderm	-	3R	Intron CG7855 (opposite strand)
HDC13470	BK002532	Cellular blastoderm subset segmentally repeated, ectoderm, embryonic foregut, embryonic hindgut	dp, EST	3R	-
HDC13644	BK002551	Embryonic midgut, anal pads	-	3R	-
HDC13905	BK002563	Trunk mesoderm anlage, embryonic midgut	dp	3R	-
HDC14221	BK002623	Ventral ectoderm anlage, posterior endoderm anlage	dp	3R	Intron CG31243 (opposite strand)
HDC14231	BK002626	Maternal, salivary glands	dp, EST	3R	Short overlap with TE19396
HDC14493	BK002672	Dorsal vessel	ag, dp	3R	Intron CG31175
HDC15090	BK002773	Maternal	dp	3R	-
HDC15681	BK002831	Weak, ubiquitous at 4-8 h	dp, EST	3R	-
HDC15728	BK002837	Maternal	dp	3R	-
HDC16092	BK002888	Weak, ubiquitous at 4-8 h	dp	3R	-
HDC16243	BK002914	Anterior endoderm anlage, anterior midgut primordium, posterior midgut primordium	dp	3R	-
HDC16874	BK002959	Yolk nuclei, anterior endoderm anlage, embryonic midgut, subset of cells	ag	X	-
HDC16879	BK002961	Invaginating cells (hemocytes?/oenocytes?)	dp	X	-
HDC17351	BK003012	Embryonic gut	-	X	-
HDC18148	BK003079	Weak, ubiquitous at 4-8 h	-	X	-
HDC18326	BK003102	Weak, ubiquitous at 4-8 h	dp	X	Intron CG1691
HDC18410	BK003108	Weak, ubiquitous at 4-8 h	dp	X	-
HDC19378	BK003172	Weak, ubiquitous at 4-8 h	dp	3R	-
HDC19530	BK003190	Weak, ubiquitous at 4-8 h	-	X	-
HDC19643	BK003204	Midgut primordium, embryonic midgut	ag	X	Intron CG32541 (opposite strand)
HDC19645	BK003205	Cuprophilic cells	-	X	-

For 40% of the novel genes tested we detected an expression pattern during embryonic development. Any overlap with regions conserved in D. pseudoobscura (dp), in A. gambiae (ag) or with D. melanogaster ESTs (EST) is listed. Note that the novel genes showing distinct in situ hybridization patterns are not enriched for conservation. With minimal overlap requirements applied, the numbers are consistent with those obtained for all Heidelberg Predictions (expressed and unexpressed) as described in the computational analysis of the combined annotation.
Hild et al. Genome Biology 2003 5:R3 doi:10.1186/gb-2003-5-1-r3

Table 4