Figure 1.

Identified proteins derived from the microsomes of AD and AS cells ordered by ratios of abundance. (a) Plot of the natural log d8/d0 ratio of the total number of ICAT reagent-labeled proteins derived from microsomes of 72 h AD (d0, light ICAT isotope reagent) and AS (10 nM R1881(methyltrienolone)) (d8, heavy ICAT isotope reagent) cells. Red and green represent androgen-induced and repressed protein abundance changes. A total of 1,064 proteins were identified and quantified (detailed in Materials and methods). (A list of all proteins is contained in panel 1 (pages 1-58) in Additional data file 1.) (b) PSA polypeptide sequence. Bold italics denote tryptic, cysteine-containing peptides. (c) Observed ICAT reagent-labeled PSA peptides. The number after each peptide sequence denotes the number of times that a peptide was identified. (d) PSA expression in the microsomes of AS cells. (e-l) Comparative immunoblot analysis of AD (d0) and AS (d8) microsomal extracts probed with antibodies to PSA, TMPRS22, FAS, LDLR, β-catenin, HSP60, MST-3 and FasL, respectively. Mass spectrometric d0/d8 ratios as displayed by the XPRESS quantification software [16] are shown below each blot. The asterisk denotes the cleaved processed form of the TMPRS22 protease [19].