Table 1

The estimated half-lives of 22 labile proteins

Clone

Accession number

Gene description

Stability

Estimated half-life (h)

2

BC005843

Similar to SH3-containing protein

Short-lived

2

3

AF176555

A-kinase anchoring protein

Short-lived

3

4

AF209502

Calpain

Short-lived

1

5

U87277

Splicing factor SRp30c

Short-lived

0.5

9

BC000804

Adaptor-related protein complex

Short-lived

2

10

BC011384

ATP synthase, H⁺ transporting

Short-lived

2

12

BC005380

Apolipoprotein A-I

Short-lived

2

14

BC007513

H19, imprinted maternally expressed

Short-lived

2

18

AP000014

Function unknown

Short-lived

2

19

M69013

Guanine-nucleotide-binding regulatory protein

Short-lived

0.5

21

BC003128

CGI-89 protein

Short-lived

2

22

AL109795

Function unknown

Short-lived

2

23

X89399

Ins(1,3,4,5)P₄-binding protein

Short-lived

2

25

BC057397

Heat shock 70 kDa protein 1A

Short-lived

2

26

NM_015416

Cervical cancer 1 proto-oncogene protein p40

Short-lived

2

27

AF248272

Gag-Pro-Pol precursor protein gene.

Short-lived

3

28

X07868

Insulin-like growth factors II

Short-lived

2

29

NM_000062

Serine (or cysteine) proteinase inhibitor

Short-lived

2

30

AF068706

Gamma2-adaptin (G2AD)

Short-lived

2

32

AK054590

Function unknown

Short-lived

3

33

BC000404

Thyroid hormone receptor interactors 13

Short-lived

2

The clones were recovered as described in the text and their half-lives were estimated by FACS-based analysis of CHX chase kinetics. All 22 clones were partially sequenced and BLAST analysis performed to identify similar protein sequences in the NCBI public database.

Jiang et al. Genome Biology 2004 5:R81   doi:10.1186/gb-2004-5-10-r81