Genome-wide mutagenesis of Zea mays L. using RescueMu transposons
- Equal contributors
1 Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA
2 Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA
3 Department of Statistics, Iowa State University, Ames, IA 50011, USA
Genome Biology 2004, 5:R82 doi:10.1186/gb-2004-5-10-r82Published: 23 September 2004
Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.