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Open Access Method

Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

John Fernandes1, Qunfeng Dong2, Bret Schneider1, Darren J Morrow1, Guo-Ling Nan1, Volker Brendel23 and Virginia Walbot1*

Author Affiliations

1 Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA

2 Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA 50011, USA

3 Department of Statistics, Iowa State University, Ames, IA 50011, USA

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Genome Biology 2004, 5:R82  doi:10.1186/gb-2004-5-10-r82

Published: 23 September 2004

Abstract

Derived from the maize Mu1 transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.