Figure 3.

Identification of an HMPA-induced deletion at the CG17367 locus. (a) Electropherograms of the primary positive pool (top), of one of the four wild-type F2 flies (center), and of a mutant F2 fly (bottom) are shown. The x-axis shows fragment size in bp; the y-axis, arbitrary intensity units. For unknown reasons the deletion allele runs faster when amplified from pool DNA than from a heterozygous fly. (b) Genomic organization of CG17367/LNK. Coding (filled rectangles) and noncoding (open) exons are shown and primer-binding sites are indicated by the half arrows. The deletion/insertion is situated 16 bp 5' to a splice site of the first intron, as can be ascertained from the sequence alignment.