Method
A genome annotation-driven approach to cloning the human ORFeome
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* Corresponding author: Ian Dunham id1@sanger.ac.uk
1 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK
2 MRC Rosalind Franklin Centre for Genomics Research (formerly MRC UK Human Genome Mapping Resource Centre), Hinxton, Cambridge, CB10 1SB, UK
3 Current address: Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK
4 Current address: Centro Nacional de Biotecnología (CNB), Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain
5 Current address: Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2XY, UK
6 Current address: Department of Biochemistry, Sanger Building, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK
Genome Biology 2004, 5:R84 doi:10.1186/gb-2004-5-10-r84
Published: 30 September 2004Additional files
Additional data file 1:
The 278 successfully cloned ORFs
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Additional data file 2:
Enzymes and templates used to amplify ORFs
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Additional data file 3:
The sequence variation between ORF clone and genomic sequence
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Additional data file 4:
The nested oligonucleotide primers designed for the 398 targeted genes
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Additional data file 5:
The results of nonparametric ANOVA (Kruskal-Wallis Test) for chromosome 22 genes isolated as cDNA by the method described here only (SANGER), found in the cDNA collections only (OTHER), isolated by both ourselves and the cDNA collections (BOTH) or not isolated (NOT)
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