Open Access Method

A genome annotation-driven approach to cloning the human ORFeome

John E Collins1, Charmain L Wright1, Carol A Edwards13, Matthew P Davis1, James A Grinham1, Charlotte G Cole1, Melanie E Goward1, Begoña Aguado24, Meera Mallya25, Younes Mokrab16, Elizabeth J Huckle1, David M Beare1 and Ian Dunham1*

Author Affiliations

1 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK

2 MRC Rosalind Franklin Centre for Genomics Research (formerly MRC UK Human Genome Mapping Resource Centre), Hinxton, Cambridge, CB10 1SB, UK

3 Current address: Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK

4 Current address: Centro Nacional de Biotecnología (CNB), Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

5 Current address: Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2XY, UK

6 Current address: Department of Biochemistry, Sanger Building, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK

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Genome Biology 2004, 5:R84  doi:10.1186/gb-2004-5-10-r84

Published: 30 September 2004

Additional files

Additional data file 1:

The 278 successfully cloned ORFs

Format: DOC Size: 275KB Download file

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Open Data

Additional data file 2:

Enzymes and templates used to amplify ORFs

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Additional data file 3:

The sequence variation between ORF clone and genomic sequence

Format: DOC Size: 297KB Download file

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Additional data file 4:

The nested oligonucleotide primers designed for the 398 targeted genes

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Additional data file 5:

The results of nonparametric ANOVA (Kruskal-Wallis Test) for chromosome 22 genes isolated as cDNA by the method described here only (SANGER), found in the cDNA collections only (OTHER), isolated by both ourselves and the cDNA collections (BOTH) or not isolated (NOT)

Format: DOC Size: 32KB Download file

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Open Data

Additional data file 6:

A list of the 278 cloned ORFs

Format: FA Size: 376KB Download file

Open Data

Additional data file 7:

A list of the 398 target ORFs

Format: FA Size: 622KB Download file

Open Data