Table 3

Replication timing of artificial chromosomes

Artificial chromosomes

Replication timing

Early: 0-2 h S

Mid: 2-4 h S

Mid: 4-6 h S

Late: 6-8 h S

Line

Chromatin composition*

Replication timing^†

L

U

L

U

L

U

L

U

17-E29

Euchromatin

Early/mid

15

10

4

15

1

29

0

26

17-D34

Euchromatin

Early/mid

16

11

26

13

0

31

0

32

17-B12

Euchromatin/heterochromatin

Mid

2

24

14

15

9

19

0

30

17-C20

Euchromatin/heterochromatin

Mid

0

56

20

57

0

57

0

57

Controls

17 cen

Heterochromatin

Mid

5

182

27

166

46

209

7

236

Yq

Heterochromatin

Mid/late

0

138

1

166

70

116

118

81

The number of either labeled (L) or unlabeled (U) artificial chromosomes in lines 17-E29, 17-D34, 17-B12 or 17-C20 or host control 17 centromere regions (17 cen) or Y long arm sequences (Yq) following BrdU detection at 2 h intervals in S phase is indicated in columns early, mid or late S phase. *Chromatin composition of artificial chromosomes in the four lines indicated or control host 17 centromere or Yq regions (see Table 2). Euchromatin: euchromatin present; heterochromatin depleted. Euchromatin/heterochromatin: both euchromatin and heterochromatin present. Heterochromatin: predominantly heterochromatin; euchromatin depleted. ^†Predominant phase in S phase during which replication occurs: early/mid: first half (0-4 h) of S phase; mid S phase (2-6 h into S phase); mid to late S (4-8 h into S phase). Pooled data from all experiments were used to generate the numbers for the controls.

Grimes et al. Genome Biology 2004 5:R89   doi:10.1186/gb-2004-5-11-r89