Table 2 |
|||||
|
Genes affiliated to sulfur metabolism |
|||||
|
Gene |
Open reading frame |
AsIII |
Cd2+ |
H2O2 |
Enzyme |
|
|
|||||
|
Fold change |
|||||
|
Sulfate assimilation |
|||||
|
MET3 |
YJR010W |
5.0-20.0 |
10.8 |
-1.0-3.0 |
ATP sulfurylase |
|
MET14 |
YKL001C |
2.0-14.0 |
9.6 |
-2.0-3.0 |
APS kinase |
|
MET16 |
YPR167C |
2.0-12.0 |
9.1 |
NC |
PAPS reductase |
|
MET22 (0.5 h, 1 mM AsIII) |
YOL064C |
3.3 |
2.5 |
NC |
Diphosphonucleoside phosphohydrolase |
|
MET10 |
YFR030W |
3.0-5.0 |
5.2 |
NC |
Sulfite reductase alpha |
|
MET5/ECM17 |
YJR137C |
2.0-5.0 |
4.5 |
-2.0-4.0 |
Sulfite reductase beta |
|
MET1/20 |
YKR069W |
NC |
6 |
NC |
Uroporphyrinogen III methylase |
|
MET8 |
YBR213W |
NC |
7 |
-2.0-4.0 |
Siroheme synthase |
|
Sulfide incorporation and transulfuration pathways |
|||||
|
MET2 |
YNL277W |
4.0-3.0 |
4.6 |
NC |
Homoserine transacetylase |
|
MET25/17 |
YLR303W |
NC |
4.8 |
NC |
O-Acetylhomoserine sulfhydrylase |
|
STR4/CYS4 |
YGR155W |
2.0-2.7 |
2.5 |
-2.0-3.0 |
Cystathionine B-synthase |
|
STR1/CYS3 (4 h, 100 μM AsIII) |
YAL012W |
-3.5 |
13.4 |
NC |
Cystathionine-lyase |
|
STR3 |
YGL184C |
2.2-3.9 |
13.5 |
2.0-4.0 |
Cystathionine G-synthase |
|
YFR055W |
YFR055W |
-9.0-5.0 |
-1.1 |
NC |
Cystathionine-lyase |
|
Methionine and AdoMet biosynthesis |
|||||
|
MET6 |
YER091C |
1.0-3.5 |
NC |
-2.0-5.0 |
N5-Methyltetrahydrofolate homocysteine transferase |
|
MET7 |
YOR241W |
NC |
-1.6 |
NC |
Tetrahydrofolyl polyglutamate synthase |
|
MET13 |
YGL125W |
NC |
NC |
-1.0-3.0 |
Methylene tetrahydrofolate reductase |
|
SAM1 (4 h, 100 μM AsIII) |
YLR180W |
2.5 |
NC |
-2.0-9.0 |
AdoMet synthetase |
|
SAM2 (4 h, 100 μM AsIII) |
YDR502C |
3.8 |
NC |
-2.0-4.0 |
AdoMet synthetase |
|
MHT1 |
YLL062C |
5.0-2.8 |
10.6 |
NC |
S-methylmethionine: homocysteine S-methyltransferase |
|
Sulfur compound uptake |
|||||
|
SUL1 |
YBR294W |
5.4-2.4 |
20 |
NC |
Sulfate transporter |
|
SUL2 |
YLR092W |
2.5-2.8 |
3 |
NC |
Sulfate transporter |
|
MUP1 (2 h and 4 h, 100 μM AsIII) |
YGR055W |
5.0-14.0 |
2 |
-1.0-4.0 |
Methionine permease, high affinity |
|
MUP3 |
YHL036W |
8.0-7.0 |
7 |
Methionine permease, low affinity |
|
|
Regulatory genes |
|||||
|
MET4 (2 h, 100 μM AsIII) |
YNL103W |
2 |
1.5 |
NC |
bZIP |
|
MET28 |
YIR017C |
NC |
5 |
NC |
bZIP |
|
CBF1 |
YJR060W |
NC |
-2 |
NC |
bHLH |
|
MET30 |
YIL046W |
5.0-1.6 |
7 |
NC |
WD40 repeats F box |
|
MET31 |
YPL039W |
NC |
1 |
-1.0-7.0 |
Zinc finger |
|
MET32 |
YDR253C |
6.0-3.6 |
14 |
-1.0-4.0 |
Zinc finger |
|
|
|||||
|
Arsenic treatment stimulates a sulfur response in yeast. Gene expression data comparisons between arsenic, cadmium, and H2O2-treated Saccharomyces cerevisiae reveal arsenic and cadmium mediated sulfur responses, but none with hydrogen peroxide. AsIII column, 2 h, 100 μM and 0.5 h, 1 mM (combined biological replicates), unless noted; cadmium column, 1 h, 1 mM [63]; H2O2 column, 10, 20, 40, 60, 120 min, 400 μM [62]. Numbers in ordinary typeface denote induction; (-) and italicized numbers denote repression; NC, no change. |
|||||
|
Haugen et al. Genome Biology 2004 5:R95 doi:10.1186/gb-2004-5-12-r95 |
|||||
