Table 3

Overlap of two-hybrid data with datasets enriched for true positives

Interactions

Overlap with LexA map (N = 1,814)

Overlap with Gal4 map (N = 20,439)

Overlap in common


Yeast interlogs (hub/spoke)*

67,238

23 (1.26%)

251 (1.23%)

4

Yeast interlogs (matrix)*

244,202

25 (1.38%)

294 (1.44%)

4

Worm interlogs*

37,863

3 (0.17%)

61 (0.30%)

0

Drosophila genetic

2,751

4 (0.22%)

22 (0.11%)

1

Reference set

47

8 (0.44%)

6 (.03%)

2

Ref set (common BD)§

20

3 (0.17%)

2 (.01%)

0


*Yeast (S. cerevisiae) and worm (C. elegans) interlogs are predicted interactions between the Drosophila orthologs of interacting yeast and worm proteins; 'hub/spoke' and 'matrix' refer to the methods used to derive predicted binary interactions from the protein complex data (see Materials and methods). Genetic interactions were obtained from Flybase [27]. The Reference set includes published interactions involving any of the 106 BD proteins in the LexA data. §The subset of reference interactions involving proteins successfully used as BDs in both the Gal4 and LexA screens is also shown; no interactions from the reference set were found in both the LexA and Gal4 screens using the same BD baits. The chance of finding the indicated number of overlapping interactions with a random set of interactions was <10-4 for all but the LexA overlaps with worm interlogs (P < 0.1436) or genetic interactions (P < 0.0024) (Additional data file 6).

Stanyon et al. Genome Biology 2004 5:R96   doi:10.1186/gb-2004-5-12-r96

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