Table 1

Results of the yeast-two hybrid interaction assays with PEX5

All eukaryotic target sequences (characterized by species, SWISS-PROT or NCBI-Refseq accession number, score from the PTS1 predictor [7], carboxy-terminal sequence and description) were tested for interaction with the tetratricopeptide (TPR) repeat domain of human PEX5, except for P12687 and DHFR-SKL where the corresponding TPR domains were derived from yeast PEX5. The prokaryotic proteins were assayed using PEX5 from both yeast and human. As the estimated length of the PTS1 signal is 12 carboxy-terminal residues [13], we chose the carboxy-terminal 16-mers to be sure that we have included the complete motif-carrying segment. *A PTS1 prediction score above zero is considered predictive of a functional PTS1 signal; a score between -10 and 0 is considered a 'twilight zone' prediction. It should be noted that the negative score for the DHFR-SKL carboxyl terminus in its context is generated by the PTS1 predictor [7] solely by terms that evaluate its potential accessibility for PEX5. ^†A yeast-two hybrid assay is considered positive if the measured β-galactosidase activity is clearly greater than zero. Experience from previous test series suggests a lower limit of around 10 Miller Units per mg protein [12] for the detection of a productive interaction. The measured β-galactosidase activities (including standard deviations) range from weak (P81708, P13368) to strong (P80209, P12687).

Neuberger et al. Genome Biology 2004 5:R97   doi:10.1186/gb-2004-5-12-r97