The evolutionary distance required to confidently identify conserved binding sites varies among transcription factors. (a) Median p-values for functional binding sites for various factors at different evolutionary distances. The evolutionary distance needed to obtain a desired significance varies between factors. (b) Predicted dependence of the p-values on evolutionary distance. Specificity data and functional binding sites were obtained from the SCPD.
Moses et al. Genome Biology 2004 5:R98 doi:10.1186/gb-2004-5-12-r98