Comparison of labeling profiles in amino acids of B. subtilis mutants that were normalized by subtraction with the wild-type values obtained under the same condition, as obtained from five different medium compositions. The line deviates above (or below) the null line when an amino acid (represented by their one letter code at the top of the first panel) mass is more (or less) abundant in the mutant than in the parent. For each amino acid, the available data points are in the order of their total mass fragment. Gray areas represent the deviation of the normalized values, based on duplicate analyses of mutant and wild type. To reduce the dimension of the data for visual comparison, we excluded those values that, on average, accounted for less than 5% of the fragment pool in all mutants under a given condition.
Zamboni and Sauer Genome Biology 2004 5:R99 doi:10.1186/gb-2004-5-12-r99