Fluxome profiling by independent component analysis of B. subtilis mutants grown on a 50:50 mixture of [U-13C]- and naturally labeled sorbitol with ammonium. (a) Weights of input variables (amino-acid mass-distribution vectors) in the mixing matrix of 10 ICs. (b) Projections (on x-axis) of samples on the IC shown in (a). The vertical line is drawn to intersect the average of the wild-type values. (c) Wild-type-normalized labeling profiles. Colors are used to highlight those aspects of the amino-acid profiles that were identified by ICA as relevant for the discrimination of the samples (b) along selected components.
Zamboni and Sauer Genome Biology 2004 5:R99 doi:10.1186/gb-2004-5-12-r99