Table 3 |
|||
|
Bacterial strains |
|||
| Strain or plasmid |
Relevant genotype |
Reference or source |
|
|
|
|||
| Salmonella |
ATCC 14028s |
Wild type |
ATCC |
| JSG421 |
pmrA::Tn10d |
[21] kind gift of J.S. Gunn |
|
| LB5010 |
metA22 metE551 ilv-452 leu-3121 trpΔ2 xyl-404 galE856 hsdLT6 hsdSA29 hsdSB121 rpsL120 |
[67] |
|
| E. coli |
DH5α |
Fφ80ΔlacZM15 Δ(lacZYAargF)U169 deoP recA1 endA1 hsdR17 (rk-mk-) |
Gibco BRL |
| Plasmids |
pCRII-TOPO |
Cloning vector, AmpR |
Invitrogen |
| pFPV25 |
ColE1 mob bla promoterless gfpmut3, AmpR |
[40] kind gift of R. Valdivia and S. Falkow |
|
| pFPV25.1 |
Constitutive rpsM promoter in pFPV25, AmpR |
[40] kind gift of R. Valdivia and S. Falkow |
|
| pCMPG5611 |
pFPV25 with yibD promoter |
This work |
|
| pCMPG5612 |
pFPV25 with ybjG promoter |
This work |
|
| pCMPG5613 |
pFPV25 with STM1269 promoter |
This work |
|
| pCMPG5614 |
pFPV25 with yjdB promoter |
This work |
|
| pCMPG5621 |
pFPV25 with sseJ promoter |
This work |
|
| pCMPG5615 |
pCMPG5611 with point mutated putative PmrA motif C→A |
This work |
|
| pCMPG5616 |
pCMPG5611 with point mutated putative PmrA motif T→G |
This work |
|
| pCMPG5617 |
pCMPG5611 with point mutated putative PmrA motif T→G |
This work |
|
| pCMPG5618 |
pCMPG5611 with point mutated putative PmrA motif A→C |
This work |
|
| pCMPG5619 |
pCMPG5611 with point mutated putative PmrA motif A→C |
This work |
|
| pCMPG5620 |
pCMPG5611 with point mutated putative PmrA motif T→C |
This work |
|
|
|
|||
|
Marchal et al. Genome Biology 2004 5:R9 doi:10.1186/gb-2004-5-2-r9 |
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