Table 4 |
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|
Primers used to construct the GFP promoter fusions |
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| Name |
Sequence 5' to 3' |
Description |
|
|
||
| Amplification of promoter regions |
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| Pro-115 |
CCGAATTCTAATTCGAGTTGCTTAAAGGCGGC |
Amplification of yibD promoter region |
| Pro-116 |
CCGGATCCGCTCCCGCATTATATAACGGG |
Amplification of yibD promoter region |
| Pro-117 |
CCGAATTCGCCAATAAAAACCGCGCAGAGTG |
Amplification of mig-13 promoter region |
| Pro-118 |
CCGGATCCAGCGAGTTGTTAAGGTTTTCCAGC |
Amplification of mig-13promoter region |
| Pro-119 |
CCGAATTCGAAGATTCCGCAGAATCAACGGCC |
Amplification of aroQ promoter region |
| Pro-120 |
CCGGATCCGGTGCTGCACATCAATAAAGAACAAAG |
Amplification of aroQ promoter region |
| Pro-121 |
CCGAATTCGTATTGCATCTGGGCGGTCATCG |
Amplification of pmrC promoter region |
| Pro-122 |
CCGGATCCAGGCGATTTGCCCAAGAACAGG |
Amplification of pmrC promoter region |
| Pro-224 |
ATGAATTCGCTTCCCCATCCCAAACCACC |
Amplification of sseJ promoter region |
| Pro-225 |
ATGGATCCGGAAGGCGTGCGCTTTCTTTTATC |
Amplification of sseJ promoter region |
|
|
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|
Restriction sites in the primers are indicated in bold type. |
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|
Marchal et al. Genome Biology 2004 5:R9 doi:10.1186/gb-2004-5-2-r9 |
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