Method
FlyGEM, a full transcriptome array platform for the Drosophila community
1 Incyte Genomics, Palo Alto, CA 94304, USA
2 Laboratory of Developmental and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Room 3339, Bethesda, MD 20892, USA
3 Gene Expression Omnibus, National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20892, USA
Genome Biology 2004, 5:R19 doi:
Published: 26 February 2004Abstract
We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.
