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Open Access Method

FlyGEM, a full transcriptome array platform for the Drosophila community

Rick Johnston1, Bruce Wang1, Rachel Nuttall1, Michael Doctolero1, Pamela Edwards2, Jining Lü2, Marina Vainer1, Huibin Yue1, Xinhao Wang1, James Minor1, Cathy Chan1, Alex Lash3, Thomas Goralski1, Michael Parisi2, Brian Oliver2* and Scott Eastman1

Author Affiliations

1 Incyte Genomics, Palo Alto, CA 94304, USA

2 Laboratory of Developmental and Cellular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Room 3339, Bethesda, MD 20892, USA

3 Gene Expression Omnibus, National Center for Biotechnology Information, National Library of Medicine, Bethesda, MD 20892, USA

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Genome Biology 2004, 5:R19  doi:

Published: 26 February 2004

Abstract

We have constructed a DNA microarray to monitor expression of predicted genes in Drosophila. By using homotypic hybridizations, we show that the array performs reproducibly, that dye effects are minimal, and that array results agree with systematic northern blotting. The array gene list has been extensively annotated and linked-out to other databases. Incyte and the NIH have made the platform available to the community via academic microarray facilities selected by an NIH committee.