• Top
• Abstract
• Deposited research article

As a service to the research community, Genome Biology used to publish non-peer-reviewed articles in a 'preprint' depository to which any research can be submitted and which all individuals can access free of charge.From January 2006 Genome Biology no longer publishes new articles in this section. Any article could be submitted by authors, who have sole responsibility for the article's content. The only screening process is to ensure relevance of the preprint to Genome Biology's scope and to avoid abusive, libellous or indecent articles. Articles in this section of the journal have not been peer-reviewed. Each preprint has a permanent URL, by which it can be cited. Research submitted to the preprint depository may be simultaneously or subsequently submitted to Genome Biology or any other publication for peer review; the only requirement is an explicit citation of, and link to, the preprint in the article that is eventually published. If possible, Genome Biology will provide a reciprocal link from the preprint depository to the published article.

Deposited research article

Characterization of Indian cattle breeds, Ongole and Deoni (Bos indicus) using microsatellite markers

Muralidhar Metta¹, Sriramana Kanginakudru², Narasimharao Gudiseva¹ and Javaregowda Nagaraju²

¹Department of Animal Genetics and Breeding, College of Veterinary Science, A NG R Agricultural University, Hyderabad 500 030, India

²Centre for DNA Fingerprinting and Diagnostics, ECIL Road, Nacharam, Hyderabad 500 076, India

author email corresponding author email

Genome Biology 2004, 5:P8doi:10.1186/gb-2004-5-4-p8

This was the first version of this article to be made available publicly. A peer-reviewed and modified version is now available in full at http://www.biomedcentral.com/1471-2156/5/16

Subject areas: Genetics

The electronic version of this article is the complete one and can be found online at: http://genomebiology.com/2004/5/4/P8

Received:	1 March 2004
Posted:	4 March 2004

© 2003 BioMed Central Ltd

Abstract

Molecular characterization of cattle breeds is important for the prevention of germplasm erosion by cross breeding. The present study was carried out to characterize two Indian cattle breeds, Ongole and Deoni using microsatellite markers. Using 5 di-and 5 tri- nucleotide repeat loci, 17 Ongole and 13 Deoni unrelated individuals were studied. Of the ten loci, eight revealed polymorphism in both the breeds. The di-nucleotide repeats loci were found to be more polymorphic (100%) than tri-nucleotide repeat loci (60%). A total of 39 polymorphic alleles were obtained at 4.5 alleles per locus in Ongole and 4.1 in Deoni. The average expected heterozygosity was 0.46 (+0.1) and 0.50 (+0.1) in Ongole and Deoni breeds, respectively. The PIC values of the polymorphic loci ranged from 0.15 to 0.79 in Ongole and 0.13 to 0.80 in Deoni breeds. Six Ongole specific and three Deoni specific alleles were identified. The two breeds showed a moderate genetic relationship between themselves with a F_ST value of 0.10.

Deposited research article

Have something to say? Post a comment on this article!