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Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements

Heping Zhou1, Kerri Bouwman1, Mark Schotanus1, Cornelius Verweij2, Jorge A Marrero3, Deborah Dillon4, Jose Costa4, Paul Lizardi4 and Brian B Haab1*

Author Affiliations

1 The Van Andel Research Institute, 333 Bostwick, Grand Rapids, MI 49503, USA

2 The University of Amsterdam, Department of Molecular and Cell Biology, 1081 BT Amsterdam, The Netherlands

3 The University of Michigan Medical School, 1500 East Medical Center Drive, Ann Arbor, MI 48109, USA

4 Yale University School of Medicine, Department of Pathology, 310 Cedar Street, New Haven, CT 06510, USA

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Genome Biology 2004, 5:R28  doi:

Published: 30 March 2004

Additional files

Additional data file 1:

The net signals and backgrounds from the experiments on hydrogel slides used in figures 2 and 3

Format: XLS Size: 60KB Download file

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Additional data file 2:

The net signals and backgrounds from the experiments on nitrocellulose slides used in figures 2 and 3

Format: XLS Size: 82KB Download file

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Additional data file 3:

The averaged and normalized data used in figures 4 and 5C from set one of the indirect detection experiments

Format: TXT Size: 9KB Download file

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Additional data file 4:

The averaged and normalized data used in figures 4 and 5C from set two of the indirect detection experiment

Format: TXT Size: 9KB Download file

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Additional data file 5:

The raw ratios (not averaged or normalized) used in figures 4 and 5C from set one of the indirect detection experiments

Format: TXT Size: 23KB Download file

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Additional data file 6:

The raw ratios (not averaged or normalized) used in figures 4 and 5C from set two of the indirect detection experiments

Format: TXT Size: 24KB Download file

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Additional data file 7:

The averaged and normalized data used in figures 4 and 5C from set one of the RCA detection experiments

Format: TXT Size: 11KB Download file

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Additional data file 8:

The averaged and normalized data used in figures 4 and 5C from set two of the RCA detection experiments

Format: TXT Size: 11KB Download file

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Additional data file 9:

The raw ratios (not averaged or normalized) used in figures 4 and 5C from set one of the RCA detection experiments

Format: TXT Size: 28KB Download file

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Additional data file 10:

The raw ratios (not averaged or normalized) used in figures 4 and 5C from set two of the RCA detection experiments

Format: TXT Size: 29KB Download file

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Additional data file 11:

The Elisa values that were used for normalization of the data (Additional data file

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Additional data file 12:

The antibodies used in this study

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Additional data file 13:

The sequences for the primers, circles and decorators used in this study

Format: DOC Size: 21KB Download file

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