Enriching for direct regulatory targets in perturbed gene-expression profiles
1 Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM 87131, USA
2 Department of Biology, University of New Mexico, Albuquerque, NM 87131, USA
3 Current address: DOE Joint Genome Institute, 2800 Mitchell Drive, Bldg 400, Walnut Creek, CA 94596, USA
Genome Biology 2004, 5:R29 doi:Published: 30 March 2004
Here we build on a previously proposed algorithm to infer direct regulatory relationships using gene-expression profiles from cells in which individual genes are deleted or overexpressed. The updated algorithm can process networks containing feedback loops, incorporate positive and negative regulatory relationships during network reconstruction, and utilize data from double mutants to resolve ambiguous regulatory relationships. When applied to experimental data the reconstruction procedure preferentially retains direct transcription factor-target relationships.