Quality of reconstruction using double-mutant data. (a) Fraction of correct edges in reconstruction (including cycles) versus edge density using single-mutant data only (original) or including double-mutant data (refined). (b) Number of double mutants needed for accurate reconstruction of networks with different numbers of edges, as compared to the number of genes in the network, N. (c) Improvement of reconstruction as double-mutant data is added for a network with 1.2 edges per node. All synthetic networks had 500 genes and the indicated number of edges. Data in (c) is for one of the four independent networks analyzed in (a) and (b), where each data point represents the average ± standard deviation for four independent networks.
Tringe et al. Genome Biology 2004 5:R29