Maize DNA-sequencing strategies and genome organization
Department of Agronomy and Plant Genetics, and Center for Plant and Microbial Genomics, The University of Minnesota, St. Paul, MN 55108, USA
Genome Biology 2004, 5:223 doi:Published: 16 April 2004
A large amount of repetitive DNA complicates the assembly of the maize genome sequence. Genome-filtration techniques, such as methylation-filtration and high-CoT separation, enrich gene sequences in genomic libraries. These methods may provide a low-cost alternative to whole-genome sequencing for maize and other complex genomes.