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Maize DNA-sequencing strategies and genome organization

Ron J Okagaki* and Ronald L Phillips

Author Affiliations

Department of Agronomy and Plant Genetics, and Center for Plant and Microbial Genomics, The University of Minnesota, St. Paul, MN 55108, USA

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Genome Biology 2004, 5:223  doi:

Published: 16 April 2004

Abstract

A large amount of repetitive DNA complicates the assembly of the maize genome sequence. Genome-filtration techniques, such as methylation-filtration and high-CoT separation, enrich gene sequences in genomic libraries. These methods may provide a low-cost alternative to whole-genome sequencing for maize and other complex genomes.