This article has not been peer reviewed.Deposited research article
AutoPrime: selecting primers for expressed sequences
- Equal contributors
1 Divisions of Bioinformatics and Biochemistry, Swiss Institute of Bioinformatics, Klingelbergstrasse 50/70, 4056 Basel, Switzerland
2 Molecular Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
Genome Biology 2004, 5:P11 doi:10.1186/gb-2004-5-5-p11
This was the first version of this article to be made available publicly.Published: 15 April 2004
Primers designed for expressed sequences should be specific for the mRNA sequence of a gene but not yield a product from its genomic sequence. Already slight contaminations of genomic DNA can lead to incorrect measurements for sensitive methods like real-time PCR (RT-PCR).
Results and Conclusions
The software AutoPrime automates the task of generating such primers for RT-PCR experiments by combining the information from sequence databases with primer design software. Thus the software is able to cope with the high demand of RT-PCR validation experiments following the recent increase in microarray expression profiling studies. The software generates high quality primers more efficiently than possible by manual design methods and has been successfully employed for RT-PCR primer design in our as well as other laboratories.