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Open Access Software

ELXR: a resource for rapid exon-directed sequence analysis

Jeoffrey J Schageman1235*, Christopher J Horton1, Sijing Niu1, Harold R Garner1345 and Alexander Pertsemlidis125

Author Affiliations

1 Eugene McDermott Center for Human Growth and Development, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA

2 Frank M. Ryburn Jr. Cardiology Center, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA

3 Center for Biomedical Inventions, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA

4 Department of Biochemistry, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA

5 Department of Internal Medicine, University of Texas Southwestern Medical Center, Harry Hines Boulevard, Dallas, TX 75390, USA

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Genome Biology 2004, 5:R36  doi:

Published: 28 April 2004

Abstract

ELXR (Exon Locator and Extractor for Resequencing) streamlines the process of determining exon/intron boundaries and designing PCR and sequencing primers for high-throughput resequencing of exons. We have pre-computed ELXR primer sets for all exons identified from the human, mouse, and rat mRNA reference sequence (RefSeq) public databases curated by the National Center for Biotechnology Information. The resulting exon-flanking PCR primer pairs have been compiled into a system called ELXRdb, which may be searched by keyword, gene name or RefSeq accession number.