Figure 1.

Microarray experimental design. Sex biased gene expression data was derived from 44 microarray hybridizations testing 15 conditions. All experimental conditions included at least one biological replicate and most also include dye-flip hybridizations for additional replicates. Abbreviated genotypes of the samples are shown in light ellipses with the total number of replicated hybridizations in dark circles between the two samples. Full genotypes of the flies used are as follows: X/Y;tra2^- males are w^67c/BsY;tra2^B/Df(2R)trix, X/X;tra2^- sex transformed males are w^67c/+;tra2^B/Df(2R)trix, X/X;dsx^D/dsx- males are (+/+; dsx^M+R45/dsx^swe. 'tud' males and females are the progeny of homozygous tud¹ bw¹ sp¹ females mated to tud¹ bw¹ sp¹/CyO males and themselves are genotypically tud¹ bw¹ sp¹ homozygotes. Females and males are whole adult y¹ w^67c flies. Ovary, testis and no gonad samples are also derived from y¹ w^67c flies.