Figure 6.

Pathway modularity, assessed by subclustering of GI data involving genes of known function for protein trafficking or DNA replication. The 298 HU-selected deletion strains were classified, based on literature annotations of their respective gene functions, into cellular pathways (see Table 2 and Additional data file 11) and sub-clustered accordingly. Color intensity corresponds to the GI (see Figure 4 for scale). Grp refers to the cluster designation from Figure 4. (a) Growth profiles from strains carrying deletions of vesicular trafficking genes. Note the several vacuolar protein sorting mutants (vps and pep), which share a distinctive phenotypic profile, even among other genes required for protein secretion and trafficking [30]. (b-c) Clustered growth profiles from strains carrying deletions in genes important for DNA replication, divided into DNA repair and chromosome dynamics (see text for further details).