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POSaM: a fast, flexible, open-source, inkjet oligonucleotide synthesizer and microarrayer

Christopher Lausted*, Timothy Dahl, Charles Warren, Kimberly King, Kimberly Smith, Michael Johnson, Ramsey Saleem, John Aitchison, Lee Hood and Stephen R Lasky

Author affiliations

The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103, USA

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Citation and License

Genome Biology 2004, 5:R58  doi:10.1186/gb-2004-5-8-r58

Published: 27 July 2004

Abstract

DNA arrays are valuable tools in molecular biology laboratories. Their rapid acceptance was aided by the release of plans for a pin-spotting microarrayer by researchers at Stanford. Inkjet microarraying is a flexible, complementary technique that allows the synthesis of arrays of any oligonucleotide sequences de novo. We describe here an open-source inkjet arrayer capable of rapidly producing sets of unique 9,800-feature arrays.