Detection of yeast deletion-strain barcodes from genomic DNA. Barcodes from 94 strains were PCR amplified and labeled with Cy3 and with Cy5, combined, and hybridized to an inkjet array. Approximately 10-fold additional genomic DNA from YPL110C, YPL111W and YPL112C was spiked into the Cy3 PCR, resulting in a 10-fold increase in Cy3 fluorescence from the corresponding reporters (gray bars). The array was stripped and the experiment was repeated, spiking the three strains into the Cy5 PCR. This resulted in a four- to fivefold increase in Cy5 fluorescence from the corresponding reporters (white bars). The laser-scan images in the inset show the increased Cy3 (green) signal from the YPL111W spike reporter in the first hybridization and increased Cy5 (red) signal from the YPL111 W spike reporter in the second hybridization. Signal is detected from the mismatch reporter as well, but at a greatly attenuated level. The slides used as substrate in this experiment have been modified using our own method as described in Materials and methods.
Lausted et al. Genome Biology 2004 5:R58 doi:10.1186/gb-2004-5-8-r58