Table 1

Cost of materials required to make one inkjet oligonucleotide array

Item

Purchase

Unit

Price ($)

Required

Unit

Line cost ($)


Glass slides

140

each

20.85

1

each

0.15

Nano-Strip glass cleaner

3,800

ml

37.18

3.2

ml

0.03

Sodium hydroxide

1,000

g

43.15

1.25

g

0.05

Hydrochloric acid

2,500

ml

42.96

0.42

ml

0.01

Methanol

4,000

ml

18.53

6.4

ml

0.03

Epoxysilane

93

ml

21.90

0.06

ml

0.01

Rain-X silane solution

200

ml

4.00

11

ml

0.22

Isopropanol

4,000

ml

29.09

4

ml

0.03

Acetone

4,000

ml

30.69

4

ml

0.03

Slide derivatization subtotal

0.56

Nitrogen

4,000

scf

90.00

225

scf

5.06

Acetonitrile

1,000

ml

38.08

250

ml

9.52

0.02 M Iodine THF/Pyr/H2O

450

ml

36.00

32

ml

2.56

2.5% DCA in DCM

450

ml

18.00

32

ml

1.28

dA-CE phosphoramidite

250

mg

3.50

14

mg

0.20

Ac-dC-CE phosphoramidite

250

mg

3.50

14

mg

0.20

dG-CE phosphoramidite

250

mg

3.50

14

mg

0.20

dT-CE phosphoramidite

250

mg

3.50

14

mg

0.20

Tetrazole

1,000

mg

35.00

56

mg

1.96

Ammonia

2,500

ml

37.67

8

ml

0.12

Methylamine

1,000

ml

23.05

8

ml

0.18

Ethanol

3,800

ml

41.70

11

ml

0.12

3-methoxypropionitrile

1,000

ml

41.90

1.5

ml

0.06

2-methyl glutaronitrile

1,000

ml

39.15

1.5

ml

0.06

Chemical synthesis subtotal

21.71

Lifterslip coverslips

40

each

38.65

1

each

0.97

DIG Easy-Hyb solution

600

ml

228.00

0.1

ml

0.04

Control oligo (Bodipy)

12,000

pmol

250.00

10

pmol

0.21

Wash buffers

-

-

-

0.05

Test hybridization subtotal

1.26

Septa

72

each

48.18

0.05

each

0.03

Drierite

2,000

g

82.53

14

g

0.58

Syringe, 1 ml

100

each

13.62

0.3

each

0.04

Syringe, 5 ml

100

each

12.74

0.3

each

0.04

26G needles

100

each

8.37

0.3

each

0.03

Molecular sieves

500

g

31.12

0.7

g

0.04

Inkjet head

1

each

85.00

0.003

each

0.26

Exhaust filter cartridge

1

each

79.00

0.0015

each

0.12

Parafilm

250

ft

28.22

0.05

ft

0.01

Miscellaneous materials subtotal

1.14

Total

24.68


Labor, safety equipment, and waste-disposal expenses are not included. Arrays printed on commercial epoxysilane slides require an additional $7 to $12.

Lausted et al. Genome Biology 2004 5:R58   doi:10.1186/gb-2004-5-8-r58

Open Data