Table 1 |
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|
Cost of materials required to make one inkjet oligonucleotide array |
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|
Item |
Purchase |
Unit |
Price ($) |
Required |
Unit |
Line cost ($) |
|
|
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|
Glass slides |
140 |
each |
20.85 |
1 |
each |
0.15 |
|
Nano-Strip glass cleaner |
3,800 |
ml |
37.18 |
3.2 |
ml |
0.03 |
|
Sodium hydroxide |
1,000 |
g |
43.15 |
1.25 |
g |
0.05 |
|
Hydrochloric acid |
2,500 |
ml |
42.96 |
0.42 |
ml |
0.01 |
|
Methanol |
4,000 |
ml |
18.53 |
6.4 |
ml |
0.03 |
|
Epoxysilane |
93 |
ml |
21.90 |
0.06 |
ml |
0.01 |
|
Rain-X silane solution |
200 |
ml |
4.00 |
11 |
ml |
0.22 |
|
Isopropanol |
4,000 |
ml |
29.09 |
4 |
ml |
0.03 |
|
Acetone |
4,000 |
ml |
30.69 |
4 |
ml |
0.03 |
|
Slide derivatization subtotal |
0.56 |
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|
Nitrogen |
4,000 |
scf |
90.00 |
225 |
scf |
5.06 |
|
Acetonitrile |
1,000 |
ml |
38.08 |
250 |
ml |
9.52 |
|
0.02 M Iodine THF/Pyr/H2O |
450 |
ml |
36.00 |
32 |
ml |
2.56 |
|
2.5% DCA in DCM |
450 |
ml |
18.00 |
32 |
ml |
1.28 |
|
dA-CE phosphoramidite |
250 |
mg |
3.50 |
14 |
mg |
0.20 |
|
Ac-dC-CE phosphoramidite |
250 |
mg |
3.50 |
14 |
mg |
0.20 |
|
dG-CE phosphoramidite |
250 |
mg |
3.50 |
14 |
mg |
0.20 |
|
dT-CE phosphoramidite |
250 |
mg |
3.50 |
14 |
mg |
0.20 |
|
Tetrazole |
1,000 |
mg |
35.00 |
56 |
mg |
1.96 |
|
Ammonia |
2,500 |
ml |
37.67 |
8 |
ml |
0.12 |
|
Methylamine |
1,000 |
ml |
23.05 |
8 |
ml |
0.18 |
|
Ethanol |
3,800 |
ml |
41.70 |
11 |
ml |
0.12 |
|
3-methoxypropionitrile |
1,000 |
ml |
41.90 |
1.5 |
ml |
0.06 |
|
2-methyl glutaronitrile |
1,000 |
ml |
39.15 |
1.5 |
ml |
0.06 |
|
Chemical synthesis subtotal |
21.71 |
|||||
|
Lifterslip coverslips |
40 |
each |
38.65 |
1 |
each |
0.97 |
|
DIG Easy-Hyb solution |
600 |
ml |
228.00 |
0.1 |
ml |
0.04 |
|
Control oligo (Bodipy) |
12,000 |
pmol |
250.00 |
10 |
pmol |
0.21 |
|
Wash buffers |
- |
- |
- |
0.05 |
||
|
Test hybridization subtotal |
1.26 |
|||||
|
Septa |
72 |
each |
48.18 |
0.05 |
each |
0.03 |
|
Drierite |
2,000 |
g |
82.53 |
14 |
g |
0.58 |
|
Syringe, 1 ml |
100 |
each |
13.62 |
0.3 |
each |
0.04 |
|
Syringe, 5 ml |
100 |
each |
12.74 |
0.3 |
each |
0.04 |
|
26G needles |
100 |
each |
8.37 |
0.3 |
each |
0.03 |
|
Molecular sieves |
500 |
g |
31.12 |
0.7 |
g |
0.04 |
|
Inkjet head |
1 |
each |
85.00 |
0.003 |
each |
0.26 |
|
Exhaust filter cartridge |
1 |
each |
79.00 |
0.0015 |
each |
0.12 |
|
Parafilm |
250 |
ft |
28.22 |
0.05 |
ft |
0.01 |
|
Miscellaneous materials subtotal |
1.14 |
|||||
|
Total |
24.68 |
|||||
|
|
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|
Labor, safety equipment, and waste-disposal expenses are not included. Arrays printed on commercial epoxysilane slides require an additional $7 to $12. |
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|
Lausted et al. Genome Biology 2004 5:R58 doi:10.1186/gb-2004-5-8-r58 |
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