Validation of a miRNA-cleaved mRNA target. (a) Northern blot analysis of miR393a showing its expression pattern. Samples are identical to those in Figure 2b. (b) The 5' RACE product for the predicted target gene At3g26810 amplified by PCR is shown in the ethidium bromide-stained agarose gel. (c) The 5' end of the cleaved product determined by sequencing is indicated by an arrow in the miRNA:mRNA base-pairing diagram, along with the number of clones analyzed. The gene structure of At3g26810 and location of the miRNA-binding site are shown at the bottom.
Wang et al. Genome Biology 2004 5:R65 doi:10.1186/gb-2004-5-9-r65