Table 1

List of putative ER target genes

Accession number
Symbol
Gene name
Number of time points*
Gene ontology

(a) Putative ER target genes that are upregulated (59 out of 89)
AL049265

mRNA; cDNA DKFZp564F053
14
Biological_process unknown [0000004]
NM_003246
THBS1
thrombospondin 1
13
Cell adhesion [0007155]
NM_016339
Link-GEFII
Link guanine nucleotide exchange factor II
13
Neurogenesis [0007399]
U79299
OLFM1
Olfactomedin 1
13
Signal transduction [0007165]
AK026062
DNAJC1
DnaJ (Hsp40) homolog, subfamily C, member 1
12
Protein folding [0006457]
M62403
IGFBP4
Insulin-like growth factor binding protein 4
12
Signal transduction [0007165]
NM_001089
ABCA3
ATP-binding cassette, sub-family A (ABC1), member 3
12
ATP-binding cassette (ABC) transporter [0004009]
NM_003714
STC2
Stanniocalcin 2
12
Cell-cell signaling [0007267]
AF075060

Hypothetical protein DKFZp761P0423
11
Biological_process unknown [0000004]
AF271070
SLC38A1
Solute carrier family 38, member 1
11
Amino acid transport [0006865]
AK024639

Hypothetical protein FLJ20986
11
Cation transport [0006812]
AL080199

mRNA; cDNA DKFZp434E082
11
Biological_process unknown [0000004]
NM_014365
H11
Protein kinase H11
11
Translational regulation, initiation [0006446]
AF245389
GREB1†‡
GREB1 protein
10
High-affinity zinc ion transport [0006830]
NM_002894
RBBP8
Retinoblastoma binding protein 8
10
DNA repair [0006281]
NM_005067
SIAH2
Seven in absentia homolog 2 (Drosophila)
10
Ubiquitin-dependent protein degradation [0006511]
U16752
CXCL12
Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
10
Immune response [0006955]
AF086500
FZD8
Frizzled homolog 8 (Drosophila)
9
Biological_process unknown [0000004]
AF176012
JDP1
J domain containing protein 1
9
Physiological processes [0007582]
AF182416
NIF3L1
NIF3 NGG1 interacting factor 3-like 1 (S. pombe)
9
DNA methylation [0006306]
AK023772

Hypothetical protein FLJ13710
9
Developmental processes [0007275]
AK024361

Hypothetical protein FLJ14299
9
Transcription regulation [0006355]
AK025812

cDNA: FLJ22159 fis, clone HRC00251, mRNA sequence
9
Biological_process unknown [0000004]
NM_001037
SCN1B
Sodium channel, voltage-gated, type I, beta polypeptide
9
Sodium transport [0006814]
NM_003287
TPD52L1
Tumor protein D52-like 1
9
Signal transducer [0004871]
NM_003646
DGKZ
Diacylglycerol kinase, zeta 104 kDa
9
Signal transduction [0007165]
NM_014333
IGSF4
Immunoglobulin superfamily, member 4
9
Virulence [0009406]
NM_016300
ARPP-21
Cyclic AMP-regulated phosphoprotein, 21 kD
9
Biological_process unknown [0000004]
AF200341
HPYR1
Helicobacter pylori responsive 1
8
Biological_process unknown [0000004]
AK023199

cDNA FLJ13137 fis, clone NT2RP3003150, mRNA sequence
8
Biological_process unknown [0000004]
AK025571

Hypothetical protein FLJ21918
8
RNA processing [0006396]
D00265
CYCS
Cytochrome c, somatic
8
Electron transport [0006118]
NM_000926
PGR
Progesterone receptor
8
Signal transduction [0007165]
NM_001634
AMD1
S-adenosylmethionine decarboxylase 1
8
Polyamine biosynthesis [0006596]
NM_002184
IL6ST
Interleukin 6 signal transducer (gp130, oncostatin M receptor)
8
Signal transduction [0007165]
NM_003489
NRIP1
Nuclear receptor interacting protein 1
8
Transcription regulation [0006355]
NM_004878
PTGES
Prostaglandin E synthase
8
Prostaglandin metabolism [0006693]
NM_012111
C14orf3
Chromosome 14 open reading frame 3
8
Protein folding [0006457]
NM_015878
OAZIN
Ornithine decarboxylase antizyme inhibitor
8
Polyamine biosynthesis [0006596]
AK023680
PPP1R15B
Protein phosphatase 1, regulatory (inhibitor) subunit 15B
7
Biological_process unknown [0000004]
NM_001909
CTSD
Cathepsin D (lysosomal aspartyl protease)
7
Proteolysis and peptidolysis [0006508]
NM_003774
GALNT4
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4
7
Biological_process unknown [0000004]
NM_014810
CAP350
Centrosome-associated protein 350
7
Non-selective vesicle transport [0006899]
NM_016391
HSPC111
Hypothetical protein HSPC111
7
Leading strand elongation [0006272]
AK021773

cDNA FLJ11711 fis, clone HEMBA1005152, mRNA sequence
6
Biological_process unknown [0000004]
AK025766
BRI3BP
BRI3 binding protein
6
Biological_process unknown [0000004]
L23401

Human repeat region mRNA
6
Biological_process unknown [0000004]
NM_000427
LOR
Loricrin
6
Cell shape and cell size control [0007148]
NM_001932
MPP3
Membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3)
6
Signal transduction [0007165]
NM_002227
JAK1
Janus kinase 1 (a protein tyrosine kinase)
6
Protein phosphorylation [0006468]
NM_006392
NOL5A
Nucleolar protein 5A (56 kDa with KKE/D repeat)
6
Transcription [0006350]
NM_006796
AFG3L2
AFG3 ATPase family gene 3-like 2 (yeast)
6
Biological_process unknown [0000004]
NM_013324
CISH
Cytokine inducible SH2-containing protein
6
JAK-STAT cascade [0007259]
NM_016233
PADI3
Peptidyl arginine deiminase, type III
6
Protein modification [0006464]
NM_020120
UGCGL1
UDP-glucose ceramide glucosyltransferase-like 1
6
Protein modification [0006464]
AB037842
KIAA1421
KIAA1421 protein
5
RNA dependent DNA replication [0006278]
NM_001116
ADCY9
Adenylate cyclase 9
5
Signal transduction [0007165]
NM_014121

PRO0233 protein
5
Double-strand break repair [0006303]
NM_018053

Hypothetical protein FLJ10307
5
Cell death [0008219]





(b) Putative ER target genes that are downregulated (30 out of 89)
NM_012342
NMA
Putative transmembrane protein
13
Melanin biosynthesis from tyrosine [0006583]
AF039944
NDRG1
N-myc downstream regulated gene 1
11
Biological_process unknown [0000004]
AL049471

mRNA; cDNA DKFZp586N012
11
Biological_process unknown [0000004]
AK024964
NFIA
Nuclear factor I/A
9
DNA replication [0006260]
M16006
SERPINE1
Serine (or cysteine) proteinase inhibitor, clade E, member 1
9
Acute-phase response [0006953]
NM_004438
EPHA4
EphA4
9
Signal transduction [0007165]
NM_006449
CDC42EP3
CDC42 effector protein (Rho GTPase binding) 3
9
Signal transduction [0007165]
AK026298
NMES1
Normal mucosa of esophagus specific 1
8
Biological_process unknown [0000004]
D16875

Human HepG2 3' region cDNA, clone hmd1f06, mRNA sequence
8
Biological_process unknown [0000004]
NM_002237
KCNG1
Potassium voltage-gated channel, subfamily G, member 1
8
Potassium transport [0006813]
NM_003032
SIAT1
Sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
8
Protein modification [0006464]
NM_006605
RFPL2
Ret finger protein-like 2
8
Protein binding [0005515]
AK025922

Hypothetical protein FLJ22269
7
Developmental processes [0007275]
NM_000504
F10
Coagulation factor X
7
Proteolysis and peptidolysis [0006508]
NM_001139
ALOX12B
Arachidonate 12-lipoxygenase, 12R type
7
Epidermal differentiation [0008544]
NM_004354
CCNG2
Cyclin G2
7
Cell cycle checkpoint [0000075]
NM_006137
CD7
CD7 antigen (p41)
7
Humoral defense mechanism [0006959]
NM_007273
REA
Repressor of estrogen receptor activity
7
Negative control of cell proliferation [0008285]
NM_014583
LMCD1
LIM and cysteine-rich domains 1
7
Transcription factor [0003700]
NM_017572
MKNK2
MAP kinase-interacting serine/threonine kinase 2
7
Protein phosphorylation [0006468]
D49356

Human mRNA (S100C-related gene)
6
Cell cycle [0007049]
NM_001878
CRABP2
Cellular retinoic acid binding protein 2
6
Signal transduction [0007165]
NM_004388
CTBS
Chitobiase, di-N-acetyl-
6
Carbohydrate metabolism [0005975]
NM_006622
SNK
serum-inducible kinase
6
Protein phosphorylation [0006468]
AK022072

cDNA FLJ12010 fis, clone HEMBB1001635, mRNA sequence
5
Biological_process unknown [0000004]
AL137529

Hypothetical protein FLJ23751
5
Lipid metabolism [0006629]
NM_000430
PAFAH1B1
Platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45 kDa
5
Signal transduction [0007165]
NM_013332
HIG2
Hypoxia-inducible protein 2
5
Biological_process unknown [0000004]
NM_014770
CENTG1
Centaurin, gamma 1
5
Cell growth and/or maintenance [0008151]
NM_001719
BMP7
Bone morphogenetic protein 7 (osteogenic protein 1)
4
Cell growth and/or maintenance [0008151]

*Number of time points that met the 1.2-fold change in the same direction selection criteria. Genes in bold have previously been shown to be direct targets in other ER+ breast tumor cell lines (ZR75-1 and/or MCF-7). CHX treatments alone had agonistic effects on these genes; therefore their CHX sensitivity in the presence of E2 is unclear.

Lin et al. Genome Biology 2004 5:R66   doi:10.1186/gb-2004-5-9-r66