Table 3 |
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|
Validation of array data by QRT-PCR |
|||
| Gene |
MAS enrichment |
SAM enrichment |
QRT-PCR enrichment |
|
|
|||
| Highly enriched |
|||
| CG13665 |
98.9 |
8.7 |
9.0 |
| CG14957 |
95.9 |
21.9 |
23.8 |
| CG13905 |
22.6 |
17.4 |
110 |
| CG13836 |
80.6 |
30.1 |
11.7 |
| Evenly expressed |
|||
| CG17737 |
1.0 |
0.9 |
0.74 |
| CG10731 |
1.0 |
1.1 |
0.68 |
| CG8327 |
1.0 |
0.8 |
1.2 |
| Arp66 |
1.0 |
1.1 |
0.47 |
| Highly depleted |
|||
| CG13421 |
0.00 |
0.067 |
0.19 |
| CG12408 |
0.01 |
0.11 |
0.14 |
| Act88F |
0.01 |
0.14 |
0.03 |
| CG15575 |
0.01 |
0.082 |
0.008 |
|
|
|||
|
Enrichment in tubule mRNA compared to whole fly mRNA, computed from the microarray dataset with MAS 5.0 or SAM (see text), were compared with real values obtained by QRT-PCR. Four separate fly and tubule samples were run with primers for each gene, and for rp49, a ribosomal gene generally considered to be invariant. RNA quantities were calculated, and the gene:rp49 ratio calculated for each sample pair. Tubule enrichment was calculated as the (gene:rp49)tubule/(gene:rp49)fly. |
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|
Wang et al. Genome Biology 2004 5:R69 doi:10.1186/gb-2004-5-9-r69 |
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