A catalog of human cDNA expression clones and its application to structural genomics
1 Protein Structure Factory, Heubnerweg 6, 14059 Berlin, Germany
2 Max Planck Institute for Molecular Genetics, Ihnestraße 73, 14195 Berlin, Germany
3 Institute of Medical Physics and Biophysics, Charité Medical School, Ziegelstraße 5/9, 10117 Berlin, Germany
4 Alpha Bioverfahrenstechnik GmbH, Heinrich-Hertz-Straße 1b, 14532 Kleinmachnow, Germany
5 RZPD German Resource Center for Genome Research GmbH, Heubnerweg 6, 14059 Berlin, Germany
Genome Biology 2004, 5:R71 doi:10.1186/gb-2004-5-9-r71Published: 17 August 2004
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.