Figure 2.

Results of high-throughput protein purification visualised by SDS-PAGE (15% polyacrylamide) and Coomassie staining. Expression products of six hEx1 cDNA clones (1-6) are shown. W, whole cellular protein extracts; S, soluble protein cellular extracts; E, protein purified under non-denaturing conditions. M, calibration with molecular mass standards. The arrow indicates the position of lysozyme, which was added for cell lysis.