|
Protein preparation and characterization |
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| hEx1 clone* |
pQTEV subclone† |
GenBank accession number |
GenBank protein name |
Protein yield per culture volume‡ (mg/l) |
Free of aggregation§ (DLS) |
Melting temperature¶ (°C) |
|
|
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| P08596 |
758H0126 |
AAH01214 |
CGI-68 |
1.5 |
Yes |
60.3 (1) |
| H05552 |
758H0426 |
AAF76210 |
DRG-1 |
1.5 |
No |
54.8 ± 0.8 (2) |
| E10541 |
758G1126 |
AAH07250 |
Coatomer protein complex, subunit ε |
1.7 |
No |
50.8 (1) |
| E12601 |
758H1026 |
CAC37950 |
HCC-1 |
7 |
No |
No peak |
| E09507 |
758C041 |
AAB59004 |
ARA9 |
4 |
Yes |
54.7 ± 0.4 (2) |
| I07544 |
250H071 |
AAH05289 |
Thioredoxin-like |
7 |
Yes |
56.6 ± 2.9 (3) |
| N13557 |
250B082 |
AAH06346 |
Aortic preferentially expressed p. 1 |
18 |
Yes |
55.5 ± 2.2 (4) |
| J17573 |
250A012 |
AAA58682 |
Transformation-sensitive protein |
19 |
Yes |
51.1 (1) |
| G03570 |
250A062 |
AAH11960 |
Gankyrin |
3 |
Yes |
No data |
| L02591 |
250B092 |
AAH12995 |
Protein for MGC:3363 |
8 |
No |
41.4 (1) |
| C16543 |
250B062 |
AAH04430 |
Unknown |
2 |
Yes |
49.2 (1) |
| H07543 |
250B112 |
BAA78534 |
Adenylate kinase 1 |
38 |
Yes |
51.6 (1) |
| H14508 |
250F062 |
BAA04802 |
HUM22SM |
38 |
No data |
53.4 (1) |
| E07518 |
250A053 |
AAF87955 |
NBP |
7 |
Yes |
32.0 (1) |
| H08589 |
250A073 |
AAH07873 |
Unknown |
3 |
No |
No peak |
| A23602 |
250C112 |
CAB53072 |
Microtubule-associated protein |
4 |
No |
58.0 (1) |
| M05547 |
250H122 |
AAH15027 |
Prolidase |
10 |
No |
50.8 ± 1.3 (3) |
|
*RZPD clone identifiers without prefix 'MPMGp800'. †Clone in vector pQTEV used for protein production, RZPD clone identifiers without prefix 'PSFEp'. ‡Protein yield of one typical protein preparation. §As determined from measurements of purified protein preparations of 0.2-1 mg/ml. ¶Melting temperature determined by DSC. The number of independent measurements is indicated in brackets. Proteins for which no peak was observed were considered to be unfolded. | ||||||
Büssow et al. Genome Biology 2004 5:R71 doi:10.1186/gb-2004-5-9-r71 |
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