Method
Large-scale exploration of growth inhibition caused by overexpression of genomic fragments in Saccharomyces cerevisiae
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* Corresponding author: Jeanne Boyer jboyer@pasteur.fr
1 Unité de Génétique Moléculaire des Levures (URA2171 CNRS and UFR 927 Université Pierre et Marie Curie)
2 Unité de Génétique des Interactions Macromoléculaires (URA2171 CNRS), Department of Structure and Dynamics of Genomes, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris-Cedex 15, France
3 CNRS-Laboratoire de Chimie Bactérienne, 31 Chemin Joseph Aiguier, 13402 Marseille-Cedex 20, France
4 Laboratoire de Parasitologie, Faculté de Médecine St-Antoine, 27 rue de Chaligny, 75012 Paris, France
5 Unité de Génétique et Biochimie du Développement, Institut Pasteur, 25 rue du Dr Roux 75724 Paris-Cedex 15, France
Genome Biology 2004, 5:R72 doi:10.1186/gb-2004-5-9-r72
Published: 31 August 2004Abstract
We have screened the genome of Saccharomyces cerevisiae for fragments that confer a growth-retardation phenotype when overexpressed in a multicopy plasmid with a tetracycline-regulatable (Tet-off) promoter. We selected 714 such fragments with a mean size of 700 base-pairs out of around 84,000 clones tested. These include 493 in-frame open reading frame fragments corresponding to 454 distinct genes (of which 91 are of unknown function), and 162 out-of-frame, antisense and intergenic genomic fragments, representing the largest collection of toxic inserts published so far in yeast.