Stability of wild-type strain gene expression profiles. (a) Microarray expression data for two wild-type biological replicates, WT 3 and WT 5, on day 1 of the growth curve are plotted against each other. The expression data plotted are the normalized ratio of dyes Cy5- and Cy3-dCTP representing sample and reference pool, respectively. Lines showing limits of twofold change are drawn on both sides of the line of identity (identical values between datasets). The axes are log scale. Every gene for which there is data is shown (filled circles). All genes fall within the lines of twofold change. (b) As in (a), except WT 5 from day 1 of the growth curve is compared with WT 5 from day 15. Only three out of 5,050 genes, marked with arrows, changed expression by more than twofold. These genes are SPBC354.08c, encoding a hypothetical protein (2.15-fold); atp8+, F0-ATP synthase subunit 8 (2.15-fold); and cox1+, cytochrome c oxidase subunit I (2.98-fold).
Mandell et al. Genome Biology 2004 6:R1 doi:10.1186/gb-2004-6-1-r1