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Open Access Method

Microarray-based resequencing of multiple Bacillus anthracis isolates

Michael E Zwick12*, Farrell Mcafee1, David J Cutler3, Timothy D Read1, Jacques Ravel4, Gregory R Bowman1, Darrell R Galloway1 and Alfred Mateczun1

Author Affiliations

1 Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA

2 Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA

3 McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA

4 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA

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Genome Biology 2004, 6:R10  doi:10.1186/gb-2004-6-1-r10

Published: 17 December 2004

Abstract

We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10-7) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10-6). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.