Microarray-based resequencing of multiple Bacillus anthracis isolates
1 Biological Defense Research Directorate, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
2 Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA
3 McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, MD 21205, USA
4 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA
Genome Biology 2004, 6:R10 doi:10.1186/gb-2004-6-1-r10Published: 17 December 2004
We used custom-designed resequencing arrays to generate 3.1 Mb of genomic sequence from a panel of 56 Bacillus anthracis strains. Sequence quality was shown to be very high by replication (discrepancy rate of 7.4 × 10-7) and by comparison to independently generated shotgun sequence (discrepancy rate < 2.5 × 10-6). Population genomics studies of microbial pathogens using rapid resequencing technologies such as resequencing arrays are critical for recognizing newly emerging or genetically engineered strains.