Wound histology. The location of skin wounds on the back of a neonatal mouse is shown. (a) For the array studies a series of criss-cross wounds were made so that all the skin cells were as close as possible to a wound edge for collection of wound RNA. (b) For in situ hybridization studies and immunohistochemistry we made a series of three incisional wounds, so that transverse sections (broken line) contained the profiles of several wounds. Resin histology through wild-type (left-hand column) and PU.1 null wounds (right-hand column) at (c,d) 0.5 h, (e,f) 3 h, (g,h) 12 h and (i,j) 24 h post-wounding. At all stages, arrows mark the epidermal wound edges, which are seen to have met and fused in both genotypes by 24 h. An asterisk (*) marks the migrating epithelial edge. (k,l) In situ hybridization using a macrophage-specific C-fms probe reveals large numbers of macrophages recruited to the granulation tissue in frozen sections through 24 h wounds in wild-type skin (k), while none are present in equivalent tissues of the PU.1 null mouse (l). Scale bars = (c-j) 400 μM; (k,l) 250 μM.
Cooper et al. Genome Biology 2004 6:R5 doi:10.1186/gb-2004-6-1-r5